拟穴青蟹(Scylla paramamosain)六个地理群体肌肉氨基酸和脂肪酸的比较分析     

李永亮  赵欢欢  李荣华  王春琳  母昌考  宋微微  叶央芳 《海洋与湖沼》2019,50(2):465-472

本文对广西钦州(Gq)、福建霞浦(Fx)、浙江温州(Zw)、浙江三门(Zs)、浙江宁海(Zn)、浙江慈溪(Zc)等6个野生群体拟穴青蟹(Scylla paramamosain)肌肉进行了氨基酸及脂肪酸分析。共测定出19种游离氨基酸和26种脂肪酸。氨基酸含量在80.94—191.39mg/g之间, Gq为最高;总脂肪酸含量在7.97—11.38mg/g之间,Zn为最高。主要氨基酸为甘氨酸和精氨酸;主要脂肪酸为C20:5n3(EPA),占总脂肪酸含量的平均值为(22.47±5.84)%。各群体风味氨基酸和多不饱和脂肪酸含量丰富,占总氨基酸比例(TDAA/TFAA)和总脂肪酸比例(PUFA/TFA)分别为(53.62±5.43)%和(59.27±5.29)%。Zs群体的必需氨基酸含量及C22:6n3(DHA)均为最高。主成分分析结果表明:各群体的氨基酸与脂肪酸组成与含量相似性与地理距离分布具有一定的相关性,特别是Gq群体,在氨基酸与脂肪酸的分类中与其他群体区分比较明显。各群体风味氨基酸和多不饱和脂肪酸的聚类分析结果均表明:Zw、Zc与Gq群体较为接近,具有较强的甜味; Fx、Zn与Zs群体较为接近,具有较强的香味及不饱和脂肪酸营养价值。  相似文献   

玉米素和水杨酸对雨生红球藻(Haematococcus pluvialis)生长及虾青素积累的影响     

崔丹丹  杨柳  孙雪  邹昭敏  李亚鹤  张琳  徐年军 《海洋与湖沼》2018,49(3):682-691

虾青素是一种具有强抗氧化活性的类胡萝卜素,而雨生红球藻是天然虾青素的主要来源。本文以雨生红球藻(Haematococcus pluvialis)为材料,研究了植物激素玉米素和水杨酸对雨生红球藻的生长、虾青素含量及相关基因表达的影响。分别添加5种浓度的玉米素或水杨酸,结果发现0.05mg/L玉米素或25mg/L水杨酸处理5d后雨生红球藻虾青素积累最多。该浓度玉米素或水杨酸可显著提高光胁迫下藻细胞密度,最高分别达到3.4×10~5cell/mL和3.0×10~5cell/mL;同时玉米素与水杨酸组中虾青素含量显著上升,分别为1.7%和1.6%,比对照组分别增加29.2%和25.6%。玉米素缓解了高光逆境条件下光合作用基因——Rubisco大亚基(rbcL)及其活化酶(rca)、碳酸酐酶(ca)的下调表达,但对虾青素合成途径β-胡萝卜素酮化酶基因(bkt)的表达量没有显著影响;而水杨酸则相反,在胁迫后期不能缓解光合作用相关基因的下调表达,但可使bkt基因显著上调,最高可达对照组的2.5倍。本研究首次比较了玉米素和水杨酸对雨生红球藻生长和虾青素积累的影响,发现玉米素比水杨酸具有更好的促进雨生红球藻中虾青素积累的效果。  相似文献   

Biological and granulometric controls on sedimentary organic matter of an intertidal mudflat     

Lawrence M. Mayer  Peter T. Rahaim  William Guerin  Stephen A. Macko  Les Watling  Franz E. Anderson 《Estuarine, Coastal and Shelf Science》1985,20(4):491-503

A variety of measures of organic matter concentration and quality were made on samples collected from the top few mm of intertidal mudflat sediment over the course of a year, in order to assess the relative importance of biological and sedimentological influences on sedimentary organic matter. Winter and summer were times of relatively fine-grained sediment accumulation, caused by biological deposition or stabilization processes and resulting in higher organic matter concentrations. Stable carbon isotope and Br:C ratios indicated a planktonic source of bulk organic matter. Ratios of organic carbon to specific surface area of the sediments were consistent with an organic monolayer coverage of sediment grains. Correction for changing grain size during the year showed no change in the organic concentration per unit surface area, in spite of organic matter inputs by in situ primary production, buildup of heterotroph biomass and mucus coatings, and biodeposition of organic-rich seston. There were also no indications of changes in bulk organic quality, measured as hydrolyzable carbohydrates and amino acids, in response to these biological processes. It is concluded that biological processes on a seasonal time scale affect the bulk organic matter of these sediments via a modulation of grain size rather than creation or decay of organic matter.  相似文献   

北秦岭晋宁期中酸性侵入岩带地球化学特征与构造环境     

杨吉 《地球科学与环境学报》2003,25(3):16-20

北秦岭侵入岩带中的中酸性侵入岩主要形成于晋宁旋回的中晚期阶段。主要岩石类型包括辉石闪长岩、闪长岩、石英闪长岩、英云闪长岩、花岗闪长岩、斜长花岗岩、花岗岩和钾长花岗岩。岩石化学、稀土元素和微量元素地球化学研究证明,这些岩石主要形成于B型俯冲和碰撞造山的构造环境中,而钾长花岗岩形成于后造山的伸展阶段。据此,该侵入岩带揭示了北秦岭褶皱带在晋宁旋回中晚期阶段的演化过程。  相似文献   

淮山提取液替代羧甲基纤维素钠作为酸奶稳定剂的效果研究     

曾少葵  蒋志红  吴红棉 《广东海洋大学学报》2001,21(1):51-55

采用正交试验等方法对淮山提取液（DB-Ext）代替羧甲基纤维素钠(CMC)作为调配型酸奶稳定剂的效果进行了初步探讨。结果表明以原料的6.0倍水量在110℃的温度条件下，提取40min，所得的淮山提取液的粘度为3500mPa  相似文献   

EFFECT OF LIGHT INTENSITY ON THE TOTAL LIPID AND FATTY ACID COMPOSITION OF SIX STRAINS OF MARINE DIATOMS     

梁英麦康森  孙世春于道展 《中国海洋湖沼学报》2001,19(3):249-254

The effect of light intensity ( 1500 Ix and 5000 Ix) on the total lipid and fatty acid composition of six strains of marine diatoms Cylindrotheca fusiformis (B211 ), Phaeodactylum tricornutum (Bl14, Bl18 and B221) Nitzschia closterium (B222) and Chaetoceros gracilis (B13) was investigated.The total lipids of B13, Bl14, and B211 grown at 5000 Ix were lower than those grown at 1500 Ix. No evident changes were observed in Bl18, B221 and B222. Fatty acid composition changed considerably at different light intensity although no consistent correlation between the relative proportion of a single FA and light intensity. The major fatty acids of the 6 strains were 14:0, 16:0, 16:l(n- 7) and 20:5(n-3) . Cylindrothecafusiformis had high percentage of 20:4n - 6 (9.2 - 10.9% ) . The total polyunsaturated fatty acid /rl all 6 strains decreased with increasing light intensity. The percentage of the highly unsaturated fatty acid eicosapentaenoic acid (EPA) decreased with increasing light intensity in all strains except Chaetoceros gracilis.  相似文献   

Fatty Acid Composition Analyses of the DCMU Resistant Mutants of Nannochloropsis oculata (Eustigmatophyceae)     

ZHANG Jimin )  LIU Shuang )  SUN Xue )  YANG Guanpin )  ZHANG Xuecheng )  *  GAO Zhenhui ) ) North China Sea Monitoring Center  SOA  Fushun Road  Qingdao  P..R.China) College of Marine Life Sciences  Ocean University of China  Qingdao  P..R.China 《中国海洋大学学报(英文版)》2003,2(1)

1　Introduction　Nannochloropsissp .areoneofpotentialsourcesofpolyunsaturatedfattyacids ,especiallytheeicosapen taentaenoicacid (EPA ,C2 0∶5 ) ,whichisimportantforbothanimalsandhumans .　Thefattyacidcompositionofthisalgaisaffectedbyvariousenvironmentalfactors ,suchaslightintensity(Sukeniketal.,1989;Sukeniketal.,1990 ) ,imply ingthatafactorinfluencingthephotosyntheticprocessmayaffectcellularfattyacidsynthesisandtheirmetabolism .AselectionprogramofincreasingcellularEPAcontenthasbeencarriedo…  相似文献   

Design of 2-M to 6-M Liquid Mirror Containers     

Robert Content 《Experimental Astronomy》2003,15(3):123-145

A new design is proposed for large (up to 6-m) liquid mirror containers. The design uses Kevlar, foam and aluminum, as in previous designs, but with a different configuration that makes the container lighter, stronger and more rigid. The results of finite element analysis are presented, consisting in the deformations due to temperature changes and to weight, and in the security factor for each material when maximum constraints are applied. Tilt rigidity is also analyzed. They show that the composite material construction technique gives a good performance up to 6 m diameters. The figures and tables contained in this paper can be used as recipes to build containers having diameters between 2 and 6 m. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

碱消解-高效液相色谱-电感耦合等离子体质谱法测定生物样品中的甲基汞和乙基汞   总被引：7，自引：2，他引：5  

杨红霞  刘崴  李冰 《岩矿测试》2008,27(6):405-408

建立了碱消解-高效液相色谱-电感耦合等离子体质谱联用系统测定生物样品中甲基汞(MeHg)与乙基汞(EtHg)的分析方法。为提高灵敏度,选用微流量的PFA雾化器,在优化的检测条件下,MeHg及EtHg检出限可达到0.036μg/L和0.03μg/L;线性范围达到4个数量级,两条工作曲线线性相关系数为1。对1.78μg/L MeHg、1.65μg/L EtHg的混合标准溶液重复测定7次,色谱峰面积的相对标准偏差(RSD)分别为1.79%和1.44%。对标准物质BCR 464(金枪鱼)的分析结果表明,测定值与标准值基本吻合,但略低于标准值;甲基汞和乙基汞的加标回收率分别为85.9%和84.5%。高效液相色谱与质谱联用技术的高灵敏度和低检出限能够满足生物样品中汞形态定量分析的要求。  相似文献   

Experimental study on the deterioration and natural remediation of the Ariake Sea tidal mud caused by the sea laver treatment acid practice and the upward seepage of pore water liquid     

Yan-Jun Du  Song-Yu Liu  Shigenori Hayashi 《Environmental Geology》2008,55(4):889-900

This study presents a laboratory study of the following two aspects: (1) the influence of sea laver treatment acid on the geoenvironmental properties of Ariake Sea tidal mud, and (2) the natural remediation effect on the sea laver treatment acid contaminated Ariake Sea tidal mud caused by the upward seepage of pore water liquid in the mud. Firstly, the mechanisms of the transport of sea laver treatment acid in the Ariake Sea tidal mud and the generation mechanisms of the upward seepage flow in the Ariake Sea tidal mud are discussed. Secondly, a series of one-dimensional laboratory infiltration tests were carried out to investigate the deterioration of the Ariake Sea tidal mud caused by the sea laver acid treatment practice. Test results reveal that the acid treatment practice caused considerable change in the geochemical properties of the mud in terms of increase in sulfide content and decrease in pH value. After the treatment by the sea laver treatment acid, the sulfide content of the mud even exceeded the safe limit value for the benthos, which represents undesirable living condition for benthos. Thirdly, series of laboratory fresh seawater infiltration tests for the deteriorated Iida site mud were conducted to illustrate this natural remediation efficiency. It is found that with the infiltration of the fresh seawater, the sulfide content of the Iida site mud was considerably reduced and pH value increased to an acceptable range for benthos living in the tidal flat mud. With the increase in the infiltration time and the hydraulic gradient, the remediation efficiency could be increased.  相似文献